relion.protocols.protocol_sort module

class relion.protocols.protocol_sort.ProtRelionSortParticles(**kwargs)

Bases: pwem.protocols.protocol_particles.ProtParticles

Relion particle sorting protocol.

It calculates difference images between particles and their aligned (and CTF-convoluted) references, and produces Z-score on the characteristics of these difference images (such as mean, standard deviation, skewness, excess kurtosis and rotational symmetry).

convertInputStep(inputId, avgId, volId)

Create the input file in STAR format as expected by Relion. If the input particles comes from Relion, just link the file. Params:

particlesId: use this parameters just to force redo of convert if

the input particles are changed.

createOutputStep()

isInputAutoPicking()

isInputAutoRefine()

isInputClasses()

runRelionStep(params)

Execute relion steps with given params.