cryosparc2.protocols.protocol_cryosparc_helical_refinement module

class cryosparc2.protocols.protocol_cryosparc_helical_refinement.ProtCryoSparcHelicalRefine3D(**kwargs)

Bases: cryosparc2.protocols.protocol_cryorefine.ProtCryoSparcRefine3D

Reconstruct and refine a homogeneous helical assembly, with or without imposition and refinement of symmetry parameters. Helical Refinement (BETA) uses an algorithm that is conceptually similar to Egelman’s Iterative Helical Real Space Reconstruction (IHRSR) algorithm, while incorporating the same maximum likelihood framework, accelerated branch-and-bound alignment algorithm, and optional Non-Uniform regularization as used in other cryoSPARC refinement jobs.

doRunRefine()

Returns

processStep()