resmap.protocols.protocol_resmap module

class resmap.protocols.protocol_resmap.ProtResMap(**kwargs)

Bases: pwem.protocols.protocol_3d.ProtAnalysis3D

ResMap is software tool for computing the local resolution of 3D density maps from electron cryo-microscopy (cryo-EM).

Please find the manual at https://sourceforge.net/projects/resmap-latest

INPUT_HELP = ' Input volume(s) for ResMap.\n    Required volume properties:\n        1. The particle must be centered in the volume.\n        2. The background must not been masked out.\n    Desired volume properties:\n        1. The volume has not been filtered in any way (low-pass filtering, etc.)\n        2. The volume has a realistic noise spectrum.\n           This is sometimes obtained by so-called amplitude correction.\n           While a similar effect is often obtained by B-factor sharpening,\n           please make sure that the spectrum does not blow up near Nyquist.\n    '

convertInputStep(volLocation1, volLocation2)

Convert input volume to .mrc as expected by ResMap.

createOutputStep()

estimateResolutionStep(args)

Call ResMap with the appropriate parameters.