fsc3d.protocols.protocol_3dfsc module

class fsc3d.protocols.protocol_3dfsc.Prot3DFSC(**kwargs)

Bases: pwem.protocols.protocol_3d.ProtAnalysis3D

Protocol to calculate 3D FSC.

3D FSC is software tool for quantifying directional resolution using 3D Fourier shell correlation volumes.

Find more information at https://github.com/nysbc/Anisotropy

INPUT_HELP = ' Required input volumes for 3D FSC:\n        1. First half map of 3D reconstruction. Can be masked or unmasked.\n        2. Second half map of 3D reconstruction. Can be masked or unmasked.\n        3. Full map of 3D reconstruction. Can be masked or unmasked, sharpened or unsharpened.\n    '

convertInputStep()

Convert input volumes to .mrc as expected by 3DFSC.

createOutputStep()

findSphericity(fn)

run3DFSCStep()