- class spider.protocols.protocol_projmatch.DefocusGroupInfo(defocusGroup, template, ih)¶
Helper class to store some information about defocus groups like the number of particles or the docfile to be generated.
- class spider.protocols.protocol_projmatch.SpiderProtRefinement(**kwargs)¶
Reference-based refinement using SPIDER AP SHC and AP REF commands.
Iterative refinement improves the accuracy in the determination of orientations. This improvement is accomplished by successive use of more finely-sampled reference projections.
Two different workflows are suggested: with defocus groups or without (gold-standard refinement).
For more information, see: [[http://spider.wadsworth.org/spider_doc/spider/docs/techs/recon/mr.html][SPIDER documentation on projection-matching]]
Convert all needed inputs before running the refinement script.
Just run the script that was generated in convertInputStep.