Source code for tomo.viewers.viewer_tomograms

# **************************************************************************
# *
# * Authors:     Adrian Quintana (adrian@eyeseetea.com) [1]
# *
# * [1] EyeSeeTea Ltd, London, UK
# *
# * This program is free software; you can redistribute it and/or modify
# * it under the terms of the GNU General Public License as published by
# * the Free Software Foundation; either version 2 of the License, or
# * (at your option) any later version.
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# * This program is distributed in the hope that it will be useful,
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# * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
# * GNU General Public License for more details.
# *
# * You should have received a copy of the GNU General Public License
# * along with this program; if not, write to the Free Software
# * Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA
# * 02111-1307  USA
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# *  All comments concerning this program package may be sent to the
# *  e-mail address 'scipion@cnb.csic.es'
# *
# **************************************************************************


"""
This module implements visualization program
for input tomograms.
"""

import os
from distutils.spawn import find_executable
import tempfile

import pyworkflow.protocol.params as params
from pwem.viewers import EmProtocolViewer, ChimeraView
from pyworkflow.viewer import DESKTOP_TKINTER, WEB_DJANGO
import pwem.viewers as viewers
from pwem.emlib.image import ImageHandler


from tomo.protocols import ProtImportTomograms, \
    ProtImportSubTomograms
from tomo.objects import SetOfSubTomograms


TOMOGRAM_SLICES = 1
TOMOGRAM_CHIMERA = 0


[docs]class ViewerProtImportTomograms(EmProtocolViewer): """ Wrapper to visualize tomo objects with default viewers such as xmipp/showj and chimera. """ _environments = [DESKTOP_TKINTER, WEB_DJANGO] _targets = [] _label = 'viewer input tomogram' def _defineParams(self, form): form.addSection(label='Visualization of input tomograms') form.addParam('displayTomo', params.EnumParam, choices=['chimera', 'slices'], default=TOMOGRAM_CHIMERA, display=params.EnumParam.DISPLAY_HLIST, label='Display tomogram with', help='*chimera*: display tomograms as surface with ' 'Chimera.\n *slices*: display tomograms as 2D slices ' 'along z axis.\n If number of tomograms == 1, ' 'a system of coordinates is shown' ) # def visualize(self, obj, **args): # views = self._showTomogramsSlices() # if views: # for v in views: # v.show() # def _getVisualizeDict(self): return { 'displayTomo': self._showTomograms, } def _validate(self): if (self.displayTomo == TOMOGRAM_CHIMERA and find_executable(viewers.viewer_chimera.Chimera.getProgram()) is None): return ["chimera is not available. " "Either install it or choose option 'slices'. "] return [] # ========================================================================= # ShowTomograms # ========================================================================= def _showTomograms(self, paramName=None): if self.displayTomo == TOMOGRAM_CHIMERA: return self._showTomogramsChimera() elif self.displayTomo == TOMOGRAM_SLICES: return self._showTomogramsSlices() def _sourceIsOutput(self): """If source is a SetOfSubtomograms""" return isinstance(self.protocol, SetOfSubTomograms) def _getOutput(self): if self._sourceIsOutput(): # Protocol here is a SetOfSubtomograms return self.protocol elif hasattr(self.protocol, 'outputTomograms'): return self.protocol.outputTomograms elif hasattr(self.protocol, 'outputSubTomograms'): return self.protocol.outputSubTomograms def _getSetAndSampling(self): setOfObjects = self._getOutput() sampling = setOfObjects.getSamplingRate() return sampling, setOfObjects def _getExtraFolder(self, fileName): if self._sourceIsOutput(): return os.path.join(tempfile.mkdtemp(), fileName) else: return self.protocol._getExtraPath(fileName) def _showTomogramsChimera(self): """ Create a chimera script to visualize selected tomograms. """ tmpFileNameCMD = self._getExtraFolder("chimera.cxc") f = open(tmpFileNameCMD, "w") sampling, _setOfObjects = self._getSetAndSampling() count = 1 # first model in chimera is a tomogram if len(_setOfObjects) == 1: count = 2 # first model in chimera is the bild file # if we have a single tomogram then create axis # as bild file. Chimera must read the bild file first # otherwise system of coordinates will not # be in the center of the window dim = _setOfObjects.getDim()[0] tmpFileNameBILD = os.path.abspath(self._getExtraFolder("axis.bild")) viewers.viewer_chimera.Chimera.createCoordinateAxisFile(dim, bildFileName=tmpFileNameBILD, sampling=sampling) f.write("open %s\n" % tmpFileNameBILD) f.write("cofr 0,0,0\n") # set center of coordinates oldFileName = "" for tomo in _setOfObjects: fileName = tomo.getFileName() if fileName == oldFileName: continue else: oldFileName = fileName localTomo = os.path.abspath(ImageHandler.removeFileType( tomo.getFileName())) if localTomo.endswith("stk"): self.showError("Extension .stk is not supported") f.write("open %s\n" % localTomo) f.write("volume #%d style surface voxelSize %f\n" % (count, sampling)) f.write("volume #%d level 1\n" % count) count += 1 if len(_setOfObjects) > 1: f.write('tile\n') else: x, y, z = tomo.getShiftsFromOrigin() f.write("volume #2 origin %0.2f,%0.2f,%0.2f\n" % (x, y, z)) f.close() view = ChimeraView(tmpFileNameCMD) return [view] def _showTomogramsSlices(self): # Write an sqlite with all tomograms selected for visualization. sampling, setOfObjects = self._getSetAndSampling() viewParams = {viewers.showj.MODE: viewers.showj.MODE_MD} view = viewers.views.ObjectView(self._project, setOfObjects.strId(), setOfObjects.getFileName(), viewParams=viewParams) view.setMemory(viewers.showj.getJvmMaxMemory() + 2) return [view]