Predicting initial models with ModelAngelo

ModelAngelo is an automatic model building program for cryo-EM maps ([Jamali et al., 2023]). In this tutorial we show how to generate ModelAngelo models, with or without using a sequence as input, for a 3D density map using the Scipion framework.

• Get ModelAngelo initial models

• Compare initial models with already traced models

Contents

• 1. Revision History
• 2. Prelude
• 3. Introduction to Model building with ModelAngelo
• 4. Problem to solve: Human ion channel TACAN isoform induced by tumor necrosis factor alpha
• 5. General workflow followed in this tutorial to predict and compare ModelAngelo structures
• 6. Get the initial model with ModelAngelo
• 7. Comparison of ModelAngelo generated models with the published traced atomic structure

Appendices

• 1. ModelAngelo Initial Model Prediction protocol