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Mouse apoferritin image processing in Scipion 3

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This tutorial presents a complete workflow of Cryo-EM single particles inside Scipion 3. It demonstrates the combination of different EM-packages with high focus on Xmipp highres.

Guide:

apoferritin image processing tutorial in scipion3

You may download a fully solved compressed project (Example_10248_Scipion3.tgz) of this tutorial from here.

Workflow: download

Relion in Scipion

This tutorial is focused on using Relion 4 single particle workflow inside Scipion. It follows the processing pipeline described for Relion 4.0 tutorial with Beta-galactosidase data.

Guide: scipion_tutorial_relion.pdf

Full SPA processing video tutorial

4 video tutorials in a list to go from movies to a 3D volume using betagalactosidase data.

Part1: Micrograph processing

Part1: Micrograph processing

Part2: Particle picking

Part2: Particle picking

Part3: 2D classification and initial volume

Part3: 2D classification and initial volume

Part4: 3D Reconstruction and validation

Part4: 3D Reconstruction and validation

Take a look at our tutorial videos in the Scipion youtube channel.

Know more about the theory and practice behind Image Processing in EM.

Initial model estimation

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This tutorial uses different methods to obtain an initial 3D map. Methods covered are RCT, Ransac, Eman and Reconstruct Significant.

Guide: scipion_tutorial_initialvolume.pdf

Initial volume validation by SAXS

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This tutorial provides a guide to the validation of the initial volume estimations using SAXS curves in Scipion.

Guide: scipion_tutorial_SAXS.pdf

Localized reconstruction in Scipion

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In this tutorial, we provide a step-by-step guide for handling symmetry mismatches in single-particle analysis using a new plugin called LocalRec (Ilca et al. 2015) in Scipion (Abrishami et al. 2020).

Guide: scipion_tutorial_localrec.pdf