Introduction to Scipion
This tutorial provides a quick introduction to processing with Scipion. It is designed to take a short time while illustrating the main concepts. The tutorial uses 3 micrographs from the Grigorieff BPV dataset. A final virus 3D map is obtained.
Initial model estimation
This tutorial uses different methods to obtain an initial 3D map. Methods covered are RCT, Ransac, Eman and Reconstruct Significant.
Mix-and-match in Scipion
This tutorial presents a more complete workflow of Cryo-EM single particles inside Scipion. It is focused on demonstrate the combination of different EM-packages. This tutorial follows the processing pipeline described for Relion 1.3 tutorial with Beta-galactosidase data.
You may download a fully solved project of this tutorial from here.
Full processing video tutorial
Know more about the theory and practice behind Image Processing in EM.
Mouse apoferritin image processing in Scipion 3.0.0
This tutorial presents a complete workflow of Cryo-EM single particles inside Scipion 3. It demonstrates the combination of different EM-packages with high focus on Xmipp highres.
You may download a fully solved compressed project (Example_10248_Scipion3.tgz) of this tutorial from here.
Initial volume validation by SAXS
This tutorial provides a guide to the validation of the initial volume estimations using SAXS curves in Scipion.