tomo3D.protocols.protocol_particle_pick_consensus module¶
Consensus picking protocol
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class
tomo3D.protocols.protocol_particle_pick_consensus.
ProtTomoConsensusPicking
(**args)[source]¶ Bases:
tomo.protocols.protocol_base.ProtTomoPicking
Protocol to estimate the agreement between different particle picking algorithms. The protocol takes several Sets of Coordinates calculated by different programs and/or different parameter settings. Let’s say: we consider N independent pickings. Then, a coordinate is considered to be a correct particle if M pickers have selected the same particle (within a radius in pixels specified in the form).
If you want to be very strict, then set M=N; that is, a coordinate represents a particle if it has been selected by all particles (this is the default behaviour). Then you may relax this condition by setting M=N-1, N-2, …
If you want to be very flexible, set M=1, in this way it suffices that 1 picker has selected the coordinate to be considered as a particle. Note that in this way, the cleaning of the dataset has to be performed by other means (screen particles, 2D and 3D classification, …).
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FN_PREFIX
= 'consensusCoords_'¶
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outputName
= 'consensusCoordinates'¶
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tomo3D.protocols.protocol_particle_pick_consensus.
consensusWorker
(coords, vesicles, trMats, consensus, consensusRadius, posFn, jaccFn=None, mode=0)[source]¶ - Worker for calculate the consensus of N picking algorithms of
- M_n coordinates each one.
coords: Array of N numpy arrays of M_n coordinates each one. consensus: Minimum number of votes to get a consensus coordinate consensusRadius: Tolerance to see two coordinates as the same (in pixels) posFn: Where to write the consensus coordinates jaccFN: Where to write the Jaccard index per micrograph