This protocol subtracts a subtomogram average to a SetOfSubtomograms, which are internally aligned and numerically adjusted in order to obtain reliable results. The adjustment and subtraction is perfomed by xmipp_volume_subtraction program. A mask can be provided if the user wants to perform the subtraction in a determined region.
Align subtomograms to be in the same orientation as the reference
Subtract reference to each of the subtomogram in the input Set