relion.protocols.protocol_subtract module

class relion.protocols.protocol_subtract.ProtRelionSubtract(**args)[source]

Bases: pwem.protocols.protocol_particles.ProtOperateParticles

Signal subtraction protocol of Relion.

Subtract volume projections from the experimental particles. The particles must have projection alignment in order to properly generate volume projections.

IS_GT30()[source]
convertInputStep()[source]

Write the input images as a Relion star file.

createOutputStep()[source]
subtractStep()[source]